![]() ![]() A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Purity/SpecificityĪnti-GAPDH Antibody is directed against human GAPDH protein. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Application NoteĪnti-GAPDH Antibody has been tested for use in ELISA and western blotting. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. A loading control antibody is critical for the correct interpretation of your western blot. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. Signal intensity was normalized to the tubulin signal.GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. Normalized signal intensity of GAPDH from whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against GAPDH (1 & 2) or a scrambled siRNA (-). Western blot analysis of whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against GAPDH (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti GAPDH antibody ( VPA00187) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, 1705046) and hFAB rhodamine anti-tubulin primary antibody ( 12004166) and visualized on the ChemiDoc MP with a 300 second (chemiluminescent channel) and 60 second exposure. Arrow points to GAPDH (molecular weight 37 kDa). HRP conjugated Tidyblot ( STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc Touch Imaging System. Rabbit anti GAPDH antibody ( VPA00187) was used at 1/1000 in lanes 1-5. ![]() 8.75 μl Jurkat whole cell lysate (WCL) was run in lanes 5 & 11 while Precision Plus Protein Prestained Standards were run in lane 6. IP was performed on Jurkat cell lysates using 10 µg (lanes 1 & 7), 5 µg (lanes 2 & 8), 1 µg (lanes 3 & 9) Mouse anti GAPDH antibody (MCA4739), and 10 μg Mouse IgG1 Negative Control (lanes 4 & 10) ( MCA1209).Ĩ.75 μl of each IP was loaded onto an AnykD Criterion TGX Stain-Free gel. ![]() Western blot analysis of GAPDH IP samples. Western blot analysis of whole cell lysates probed with GAPDH antibody ( VPA00187) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |